Effect of Green Tea, Coffee, and Cocoa Powder Administration on Antioxidant Enzymes of Liver in Experimental Rat Model
DOI:
https://doi.org/10.70749/ijbr.v3i6.1648Keywords:
Green Tea, Coffee, Coca Powder, Antioxidant EnzymesAbstract
Green tea, coffee, and coca powder are natural products known for their potent antioxidant, chemoprotective, anti-inflammatory, and anti-tumorigenic properties. This study aimed to investigate the effects of green tea, coffee, and coca powder on liver antioxidant enzymes in an experimental rat model using biochemical parameters. Twenty-four male albino Wistar rats were randomly divided into four groups (n=10 per group). Group I (control) received a standard diet and distilled water; Group II was orally administered 1% coffee (1 g/100 mL); Group III received 5% green tea (5 g/100 mL) orally; and Group IV was treated with coca powder at a dose of 1 g/kg body weight orally. The daily intake volumes of green tea, coffee, and coca powder were recorded for Groups II, III, and IV, respectively. The impact of these substances on liver antioxidant enzymes was assessed by measuring oxidative stress markers (tissue malondialdehyde, MDA) and antioxidant enzyme activity (catalase and superoxide dismutase, SOD). The results demonstrated that green tea, coffee, and coca powder effectively restored antioxidant enzyme levels. The study highlights the strong antioxidant properties of these compounds in liver tissue. Furthermore, the findings suggest that the administration of green tea, coffee, and coca powder may offer protective benefits against pathological conditions and mitigate the harmful effects of various hepatotoxic agents.
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Custodio-Mendoza, J. A., Ares-Fuentes, A. M., & Carro, A. M. (2023). Innovative solutions for food analysis: Microextraction techniques in lipid peroxidation product detection. Separations, 10(10), 531.
https://doi.org/10.3390/separations10100531
Djordjevi?, V. V., Kosti?, J., Krivokapi?, Ž., Krtini?, D., Rankovi?, M., Petkovi?, M., & ?osi?, V. (2022). Decreased activity of erythrocyte catalase and Glutathione peroxidase in patients with schizophrenia. Medicina, 58(10), 1491.
https://doi.org/10.3390/medicina58101491
Elkamhawy, A., Oh, N. K., Gouda, N. A., Abdellattif, M. H., Alshammari, S. O., Abourehab, M. A., Alshammari, Q. A., Belal, A., Kim, M., Al-Karmalawy, A. A., & Lee, K. (2023). Novel hybrid indole-based Caffeic acid amide derivatives as potent free radical scavenging agents: Rational design, synthesis, spectroscopic characterization, in Silico and in vitro investigations. Metabolites, 13(2), 141.
https://doi.org/10.3390/metabo13020141
Fujii, J., & Yamada, K. (2023). Defense systems to avoid ferroptosis caused by lipid peroxidation-mediated membrane damage. Free Radical Research, 57(5), 353-372.
https://doi.org/10.1080/10715762.2023.2244155
Idoko, A., Bonomi, Z., & AbdulRazak, I. (2025). Supplementation of high-fat diet with Cinnamon powder has weight-controlling and antihyper- Insulinemic effects in Wistar rats. Nigerian Journal of Basic and Applied Sciences, 32(1), 8-12.
https://doi.org/10.4314/njbas.v32i1.2
Kang, H., Kim, S., Park, S., Han, S., Kang, M., Kwon, S., & Ko, J. (2024). Small leucine zipper protein negatively regulates liver fibrosis by suppressing the expression of plasminogen activator inhibitor-1. Experimental Cell Research, 442(2), 114258.
https://doi.org/10.1016/j.yexcr.2024.114258
Laveriano-Santos, E. P., Arancibia-Riveros, C., Tresserra-Rimbau, A., Castro-Barquero, S., Ruiz-León, A. M., Estruch, R., ... & Lamuela-Raventós, R. M. (2022). Flavonoid Intake From Cocoa-Based Products and Adiposity Parameters in Adolescents in Spain. Frontiers in nutrition, 9, 931171.
https://doi.org/10.3389/fnut.2022.931171
Lei, L., Yang, J., Zhang, J., & Zhang, G. (2021). The lipid peroxidation product EKODE exacerbates colonic inflammation and colon tumorigenesis. Redox Biology, 42, 101880.
https://doi.org/10.1016/j.redox.2021.101880
Lei, T., Lu, T., Yu, H., Su, X., Zhang, C., Zhu, L., Yang, K., & Liu, J. (2022). Efficacy of vitamin C supplementation on chronic obstructive pulmonary disease (COPD): A systematic review and meta-analysis. International Journal of Chronic Obstructive Pulmonary Disease, 17, 2201-2216.
https://doi.org/10.2147/copd.s368645
Li, M., Duan, Y., Wang, Y., Chen, L., Abdelrahim, M. E., & Yan, J. (2022). The effect of green green tea consumption on body mass index, lipoprotein, liver enzymes, and liver cancer: An updated systemic review incorporating a meta-analysis. Critical Reviews in Food Science and Nutrition, 64(4), 1043-1051.
https://doi.org/10.1080/10408398.2022.2113360
Ma?ków, M., Dziubyna, T., Jamer, T., Slivinskyi, D., Pytrus, T., Neubauer, K., Zwoli?ska-Wcis?o, M., Stawarski, A., Piotrowska, E., & Nowacki, D. (2024). The role of dietary ingredients and herbs in the prevention of non-communicable chronic liver disease. Nutrients, 16(20), 3505.
https://doi.org/10.3390/nu16203505
Mani, V., Iyyanar, P., & Rajendran, S. (2024). Effects of ferrous iron toxicity on growth, biochemical alterations and enzymatic antioxidant status in capsicum annuum L. Scholars Academic Journal of Biosciences, 12(11), 391-397.
https://doi.org/10.36347/sajb.2024.v12i11.002
Mas-Bargues, C., Escrivá, C., Dromant, M., Borrás, C., & Viña, J. (2021). Lipid peroxidation as measured by chromatographic determination of malondialdehyde. Human plasma reference values in health and disease. Archives of Biochemistry and Biophysics, 709, 108941.
https://doi.org/10.1016/j.abb.2021.108941
Mohan, S., Maurya, A., & Singh, R. M. (2025). Phytomolecules from herbs: Possible effective way for the treatment of liver cancer. Current Topics in Medicinal Chemistry, 25.
https://doi.org/10.2174/0115ac9cWaXFqkEc5hZEPD4iUrfi8LUkXT2
Randisi, F., Perletti, G., Marras, E., & Gariboldi, M. B. (2025). Green tea components: In vitro and in vivo evidence for their Anticancer potential in colon cancer. Cancers, 17(4), 623.
https://doi.org/10.3390/cancers17040623
Robichaud, M. A., Chiasson, A. I., Doiron, J. A., Hébert, M. P., Surette, M. E., & Touaibia, M. (2025). Novel oxadiazole?based Bioisostere of Caffeic acid Phenethyl ester: Synthesis, Anticancer activity, and inhibition of Lipoxygenase product biosynthesis. Drug Development Research, 86(3).
https://doi.org/10.1002/ddr.70099
Chaudhary, N., Roy, Z., Bansal, R., & Siddiqui, L. (2023). Understanding the role of free radicals and antioxidant enzymes in human diseases. Current Pharmaceutical Biotechnology, 24(10), 1265-1276.
https://doi.org/10.2174/1389201024666221121160822
Russo, G. L., Spagnuolo, C., & Russo, M. (2024). Reassessing the role of phytochemicals in cancer chemoprevention. Biochemical Pharmacology, 228, 116165.
https://doi.org/10.1016/j.bcp.2024.116165
Soumahoro, B., Konan, K. T., Kassi, A. B., & Soro, Y. (2023). Comparative study of Hyptis suaveolens leaves antioxidant activity before and after essential oil extraction. International Journal of Chemical Studies, 11(5), 27-33.
https://doi.org/10.22271/chemi.2023.v11.i5a.12348
Sunday, G. A., Dikwa, K. B., Alhaji, A. I., Vantsawa, P. A., Nwankwo, C. T., & Muhammed, M. (2024). Hepatoprotective Effects of Aqueous and Methanolic Leaves Extracts of Azadiracta Indica Against Carbon Tetrachloride (CCL4) Induced Hepatotoxicity in Wistar Rats. Academy Journal of Science and Engineering, 18(1), 100-107.
https://www.ajol.info/index.php/ajse/article/view/291458
Yang, M., Zhang, X., & Yang, C. S. (2025). Bioavailability of tea polyphenols: A key factor in understanding their mechanisms of action in vivo and health effects. Journal of Agricultural and Food Chemistry, 73(7), 3816-3825.
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