Identification of Drought Tolerant Wheat Genotypes through Multi-Index Approach under Different Irrigation Regimes

Authors

  • Habib Ur Rehman Department of Agronomy, Balochistan Agriculture College, Quetta, Pakistan
  • Nanak Khan Department of Agronomy, Balochistan Agriculture College, Quetta, Pakistan
  • Jahangir Khan PARC-Balochistan Agricultural Research and Development Centre, Quetta, Pakistan
  • Abdul Razzaq Reki Department of Agronomy, Balochistan Agriculture College, Quetta, Pakistan
  • Ghulam Rasool Department of Plant Breeding and Genetics, Balochistan Agriculture College, Quetta, Pakistan
  • Syed Riaz Ahmed PARC-Balochistan Agricultural Research and Development Centre, Quetta, Pakistan
  • Hafeezullah Department of Agronomy, Balochistan Agriculture College, Quetta, Pakistan
  • Amanullah PARC-Balochistan Agricultural Research and Development Centre, Quetta, Pakistan
  • Hassan Akhtar PARC-Balochistan Agricultural Research and Development Centre, Quetta, Pakistan
  • Muhammad Ilyas Department of Agronomy, Balochistan Agriculture College, Quetta, Pakistan

DOI:

https://doi.org/10.70749/ijbr.v4i3.2994

Keywords:

Wheat, Drought Tolerance, Genotypes, Grain Yield, Yield Stability, Drought Tolerance Indices

Abstract

Wheat is a globally important cereal crop that serves as a major staple for billions of people. However, its productivity in rainfed areas is increasingly threatened by drought (one of the most severe abiotic stresses), which is exacerbated by climate change. This study aimed to evaluate the drought tolerance of 33 exotic wheat genotypes along with seven local checks under irrigated and rainfed conditions during the 2023–24 growing season at the Balochistan Agricultural Research and Development Center (BARDC), Quetta. The experiment was conducted using a factorial RCBD design with three replicates. Grain yield under normal (Ypi) and stress (Ysi) conditions was used to compute 11 drought tolerance indices, including Mean Productivity (MP), Geometric Mean Productivity (GMP), Harmonic Mean (HARM), Stress Tolerance Index (STI), and others. Genotypes BARDC-26, BARDC-31, BARDC-24, BARDC-9, BARDC-14, and BARDC-19 consistently exhibited the highest values across multiple indices (MP, GMP, MRP, HARM, MSTIK1, MSTIK2, and REI), as well as superior grain yield under both conditions, classifying them as drought-tolerant and high-yielding. In contrast, BARDC-16, BARDC-15, BARDC-12, BARDC-8, and BARDC-2 exhibited low index values and were identified as drought-sensitive. Correlation analysis revealed strong positive relationships between both Ypi and Ysi with MP, GMP, HARM, REI, and MSTIK1, while TOL was negatively correlated with Ysi, underscoring its inefficiency in selecting drought-tolerant genotypes. PCA showed that the first two components explained 97.7% of the total variation, with drought indices such as MP, GMP, and STI contributing most to genotype differentiation. Cluster analysis grouped the genotypes into four distinct clusters, with Cluster I (including BARDC-26, BARDC-31, BARDC-24, BARDC-9, BARDC-14, BARDC-25, and BARDC-19) comprising the most stable and drought-tolerant genotypes. These findings underscore the effectiveness of multivariate drought indices in identifying stable, high-yield genotypes for both irrigated and rainfed environments. The selected genotypes offer strong potential for future breeding programs targeting drought resilience in arid and semi-arid regions of Balochistan.

Downloads

Download data is not yet available.

References

1. Abd El-Mohsen, A.A., M.A. Abd El-Shafi, E.M.S. Gheith, and H.S. Suleiman. 2015. Using different statistical procedures for evaluating drought tolerance indices of bread wheat genotypes. Advances in Agriculture and Biology 4:19‒30.

https://doi.org/10.15192/pscp.aab.2015.4.1.1930

2. Abhinandan, K., L. Skori, M. Stanic, N.M. Hickerson, M. Jamshed, and M.A. Samuel. 2018. Abiotic stress signaling in wheat–an inclusive overview of hormonal interactions during abiotic stress responses in wheat. Frontiers in Plant Science 9:734.

https://doi.org/10.3389/fpls.2018.00734

3. Ahmed, K., G. Shabbir, M. Ahmed, and K.N. Shah. 2020. Phenotyping for drought resistance in bread wheat using physiological and biochemical traits. Science of the Total Environment 729:139082.

https://doi.org/10.1016/j.scitotenv.2020.139082

4. Ahmed, S.R., M.J. Asghar, A. Hameed, M. Ghaffar, and M. Shahid. 2024. Advancing crop improvement through GWAS and beyond in mung bean. Frontiers in Plant Science 15:1436532.

https://doi.org/10.3389/fpls.2024.1436532

5. Ahmed, S.R., Z. Anwar, U. Shahbaz, M. Skalicky, A. Ijaz, M.S. Tariq, and M.M. Zafar. 2023. Potential role of silicon in plants against biotic and abiotic stresses. Silicon 15:3283‒3303.

https://doi.org/10.1007/s12633-022-02254-w

6. Al-Maskri, A., W. Al-Busaidi, H. Al-Nadabi, A. Al-Fahdi, and M.M. Khan. 2016. Effects of drought stress on wheat (Triticum aestivum L.) cv. Coolly. International Conference on Agricultural, Food and Biological Health Sciences (AFBHS-16), August:22‒24.

https://doi.org/10.17758/eap.eap816233

7. Alhag, D.D., V. Rashidi, S. Aharizad, F. Farahvash, and B. Mirshekari. 2022. The traits affecting wheat grain yield and determining tolerant genotypes using drought indices. Cereal Research Communications 50:627‒636.

https://doi.org/10.1007/s42976-021-00225-2

8. Bajkani, J.K., H. Amin, H.A. Samoon, F. Muhammad, N.A. Soomro, and S.P. Tunio. 2025. Impact assessment of zero tillage wheat sowing technology in the rice-wheat farming system of Sindh Province. Research Journal of Social Affairs 3:413‒423.

https://doi.org/10.71317/rjsa.003.02.0162

9. Bapela, T., H. Shimelis, T.J. Tsilo, and I. Mathew. 2022. Genetic improvement of wheat for drought tolerance: progress, challenges and opportunities. Plants 11:1331.

https://doi.org/10.3390/plants11101331

10. Bashir, S.S., A. Hussain, S.J. Hussain, O.A. Wani, S. Zahid Nabi, N.A. Dar, and S. Mansoor. 2021. Plant drought stress tolerance: understanding its physiological, biochemical and molecular mechanisms. Biotechnology & Biotechnological Equipment 35:1912‒1925.

https://doi.org/10.1080/13102818.2021.2020161

11. Bazzaz, M.M., A. Hossain, Q.A. Khaliq, M.A. Karim, M. Farooq, and J.A. Teixeira da Silva. 2019. Assessment of tolerance to drought stress of thirty-five bread wheat (Triticum aestivum L.) genotypes using boxplots and cluster analysis. Agriculturae Conspectus Scientificus 84:333‒345.

12. Bennani, S., N. Nsarellah, M. Jlibene, W. Tadesse, A. Birouk, and H. Ouabbou. 2017. Efficiency of drought tolerance indices under different stress severities for bread wheat selection. Australian Journal of Crop Science 11:4.

https://doi.org/10.3390/plants11070986

13. Blum, A. 2005. Drought resistance, water-use efficiency, and yield potential—are they compatible, dissonant, or mutually exclusive? Australian Journal of Agricultural Research 56:1159‒1168.

https://doi.org/10.1071/ar05069

14. Chowdhury, M.K., M.A. Hasan, M.M. Bahadur, M.R. Islam, M.A. Hakim, M.A. Iqbal, and M.S. Islam. 2021. Evaluation of drought tolerance of some wheat (Triticum aestivum L.) genotypes through phenology, growth, and physiological indices. Agronomy 11:1792.

https://doi.org/10.3390/agronomy11091792

15. Dadbakhsh, A., A. Yazdansepas, and M. Ahmadizadeh. 2011. Study drought stress on yield of wheat (Triticum aestivum L.) genotypes by drought tolerance indices. Advances in Environmental Biology 5:1804‒1810.

16. Del Pozo, A., A. Yáñez, I.A. Matus, G. Tapia, D. Castillo, L. Sanchez-Jardón, and J.L. Araus. 2016. Physiological traits associated with wheat yield potential and performance under water-stress in a Mediterranean environment. Frontiers in Plant Science 7:987.

https://doi.org/10.3389/fpls.2016.00987

17. Devi, S., V. Singh, M. Kumar, S. Yashveer, R. Kumar, S. Sharma, and M. Redhu. 2024. Assessing wheat genotype performance under heat, drought and combined stresses using multiple stress indices. Euphytica 220:164.

https://doi.org/10.1007/s10681-024-03424-0

18. Dhakal, A. 2021. Effect of drought stress and management in wheat—A review. Food and Agribusiness Management 2:62‒66.

19. Durrani, H., A. Syed, S. Gourdji, G. Murtuza, and A. Mengal. 2025. Exploring farmers’ coping strategies and adaptive measures against the extended drought in Balochistan, Province of Pakistan. Environment, Development and Sustainability 27:6663‒6682.

https://doi.org/10.1007/s10668-023-04161-5

20. El-Aty, A., S. Mohamed, K.M. Gad, Y.A. Hefny, and M.O. Shehata. 2024. Performance of some wheat (Triticum aestivum L.) genotypes and their drought tolerance indices under normal and water stress. Egyptian Journal of Soil Science 64:19‒30.

21. Farshadfar, E., and J. Sutka. 2003. Multivariate analysis of drought tolerance in wheat substitution lines. Cereal Research Communications 31:33‒40.

https://doi.org/10.1007/bf03543247

22. Fernandez, G.C. 1993. Effective selection criteria for assessing plant stress tolerance. In: Proceedings of the International Symposium on Adaptation of Vegetables and Other Food Crops in Temperature and Water Stress. Taiwan, pp. 13‒16.

23. Fischer, R.A., and J.T. Wood. 1979. Drought resistance in spring wheat cultivars. III. Yield associations with morpho-physiological traits. Australian Journal of Agricultural Research 30:1001‒1020.

https://doi.org/10.1071/ar9791001

24. Guo, J., J. Khan, S. Pradhan, D. Shahi, N. Khan, M. Avci, and M.A. Babar. 2020. Multi-trait genomic prediction of yield-related traits in US soft wheat under variable water regimes. Genes 11:1270.

https://doi.org/10.3390/genes11111270

25. Hossain, A.B.S., R.G. Sears, T.S. Cox, and G.M. Paulsen. 1990. Desiccation tolerance and its relationship to assimilate partitioning in winter wheat. Crop Science 30:622‒627.

https://doi.org/10.2135/cropsci1990.0011183x003000030030x

26. Khan, J.H.A.N., K.H. Saifullah, A.M.A. Khetran, N. Sadiq, M. Islam, A. Hanan, and A. Aziz. 2013. Tijaban-10: A drought tolerant and high yielding wheat variety for rainfed/sailaba areas of Balochistan. Pakistan Journal of Botany 45:1357‒1362.

27. Khan, S., J. Khan, N. Islam, and M. Islam. 2011. Screening and evaluation of wheat germplasm for yield, drought and disease resistance under rainfed conditions of upland Baluchistan. Pakistan Journal of Botany 43:559‒563.

28. Kumar, P., V. Dheer, P. Kumar, J. Singh, K.K. Singh, Y. Kumar, and A.K. Singh. 2024. Effect of irrigation scheduling and different sowing dates on growth and yield of wheat (Triticum aestivum L.). International Journal of Environment and Climate Change 14:155‒161.

https://doi.org/10.9734/ijecc/2024/v14i13820

29. Lan, J. 1998. Comparison of evaluating methods for agronomic drought resistance in crops. Acta Agriculturae Boreali-occidentalis Sinica 7:85‒87.

30. Lao, Y., Y. Dong, Y. Shi, Y. Wang, S. Xu, J. Xue, and X. Zhang. 2021. Evaluation of drought tolerance in maize inbred lines selected from the Shaan A group and Shaan B group. Agriculture 12:11.

https://doi.org/10.3390/agriculture12010011

31. Mahmood, N., M. Arshad, H. Kächele, H. Ma, A. Ullah, and K. Müller. 2019. Wheat yield response to input and socioeconomic factors under changing climate: Evidence from rainfed environments of Pakistan. Science of the Total Environment 688:1275‒1285.

32. Moradi, H., G.A. Akbari, S.K. Khorasani, and H.A. Ramshini. 2012. Evaluation of drought tolerance in corn (Zea mays L.) new hybrids using stress tolerance indices. European Journal of Sustainable Development 1:543.

https://doi.org/10.14207/ejsd.2012.v1n3p543

33. Mubushar, M., S. El-Hendawy, M.U. Tahir, M. Alotaibi, N. Mohammed, Y. Refay, and E. Tola. 2022. Assessing the suitability of multivariate analysis for stress tolerance indices, biomass, and grain yield for detecting salt tolerance in advanced spring wheat lines irrigated with saline water under field conditions. Agronomy 12:3084.

34. Muhammad, B., J. Khan, A.R. Reki, N. Khan, M. Sharif, M. Waris, and N. Sadiq. 2025. Wheat productivity improvement through spike fertility as selection criteria under terminal drought stress conditions. Planta Animalia 4:97‒105.

https://doi.org/10.71454/pa.004.01.0064

35. Mujtaba, S.M., S. Faisal, M.A. Khan, S. Mumtaz, and B. Khanzada. 2016. Physiological studies on six wheat (Triticum aestivum L.) genotypes for drought stress tolerance at seedling stage. Agricultural Research and Technology Open Access Journal 1:001‒005.

https://doi.org/10.19080/artoaj.2016.01.555559

36. Naveed, M., M. Aslam, S.R. Ahmed, D.K.Y. Tan, F. De Mastro, M.S. Tariq, and Y. Liu. 2025. An overview of heat stress in chickpea (Cicer arietinum L.): Effects, mechanisms and diverse molecular breeding approaches for enhancing resilience and productivity. Molecular Breeding 45:18.

https://doi.org/10.1007/s11032-025-01538-4

37. Nawaz, H., J. Khan, Z.U. Malghani, S.R. Ahmed, A.R. Reki, M.A. Khetran, and B. Ali. 2025. Evaluating the performance of wheat germplasm under drought using morphological and physiological traits. Indus Journal of Biosciences and Research 3:67‒75.

38. Naz, F., G.H. Dars, K. Ansari, S. Jamro, and N.Y. Krakauer. 2020. Drought trends in Balochistan. Water 12:470.

https://doi.org/10.3390/w12020470

39. Poudel, P.B., M.R. Poudel, and R.R. Puri. 2021. Evaluation of heat stress tolerance in spring wheat (Triticum aestivum L.) genotypes using stress tolerance indices in western region of Nepal. Journal of Agriculture and Food Research 5:100179.

40. Rijal, R.B., P. Bashyal, D.R. Yadav, K. Upadhyay, K.R. Pant, and N.R. Adhikari. 2024. Assessing drought tolerance in advance wheat genotypes using stress tolerance indices. Archives of Agriculture and Environmental Science 9:336‒344.

https://doi.org/10.26832/24566632.2024.0902019

41. Rosielle, A.A., and J. Hamblin. 1981. Theoretical aspects of selection for yield in stress and non-stress environment. Crop Science 21:943‒946.

42. Sarto, M.V.M., J.R.W. Sarto, L. Rampim, J.S. Rosset, D. Bassegio, P.F. da Costa, and A.M. Inagaki. 2017. Wheat phenology and yield under drought: a review. Australian Journal of Crop Science 11:941‒946.

https://doi.org/10.21475/ajcs.17.11.08.pne351

43. Sedghiyeh, V., F. Shekari, A. Abbasi, N. Sabaghnia, and M. Roustaii. 2025. Evaluation of drought tolerance ability in wheat genotypes through comprehensive stress indices. HAYATI Journal of Biosciences 32:117‒131.

44. Shahi, D., J. Guo, M.A. Babar, S. Pradhan, M. Avci, N. Khan, and J. Khan. 2024a. Deciphering the genetic basis of novel traits that discriminate useful and non-useful biomass to enhance harvest index in wheat. The Plant Genome 17:20512.

https://doi.org/10.1002/tpg2.20512

45. Shahi, D., J. Guo, S. Pradhan, J. Khan, M. Avci, N. Khan, and M.A. Babar. 2022. Multi-trait genomic prediction using in-season physiological parameters increases prediction accuracy of complex traits in US wheat. BMC Genomics 23:298.

46. Shahi, D., J. Guo, S. Pradhan, M. Avci, G. Bai, J. Khan, and M.A. Babar. 2024b. Genome-wide association study and genomic prediction of soft wheat end-use quality traits under post-anthesis heat-stressed conditions. Biology 13:962.

https://doi.org/10.3390/biology13120962

47. Shahrokhi, M., S.K. Khorasani, and A. Ebrahimi. 2020. Evaluation of drought tolerance indices for screening some of super sweet maize (Zea mays L. var. saccharata) inbred lines. AGRIVITA Journal of Agricultural Science 42:435‒448.

48. Shiferaw, B., M. Smale, H.J. Braun, E. Duveiller, M. Reynolds and G. Muricho. 2013. Crops that feed the world 10. Past successes and future challenges to the role played by wheat in global food security. Food Security 5:291–317.

https://doi.org/10.1007/s12571-013-0263-y

49. Shivramakrishnan, R., R. Vinoth, A.J. Arora, G.P. Singh, B. Kumar and V.P. Singh. 2016. Characterization of wheat genotypes for stay green and physiological traits by principal component analysis under drought condition. International Journal of Agricultural Sciences 12:245–251.

https://doi.org/10.15740/has/ijas/12.2/245-251

50. Singh, G., P. Kumar, V. Gupta, B.S. Tyagi, C. Singh, A.K. Sharma and G.P. Singh. 2018. Multivariate approach to identify and characterize bread wheat (Triticum aestivum) germplasm for waterlogging tolerance in India. Field Crops Research 221:81–89.

https://doi.org/10.1016/j.fcr.2018.02.019

51. Sofi, P.A., S. Shafi, B. Singh, J.P. Jaiswal, V.K. Mishra and R.R. Mir. 2021. Combined selection for productivity and resilience through modified stress tolerance indices in a HUW-234 × HUW-468 derived wheat (Triticum aestivum L.) RIL mapping population for heat stress. Electronic Journal of Plant Breeding 12:612–622.

https://doi.org/10.37992/2021.1203.087

52. Spinoni, J., G. Naumann, J. Vogt and P. Barbosa. 2015. European drought climatologies and trends based on a multi-indicator approach. Global and Planetary Change 127:50–57.

https://doi.org/10.1016/j.gloplacha.2015.01.012

53. Thanaa, H., E.A.M. Abd El-Monem and M.N.A. El-Keredy. 2019. Tolerance indices and cluster analysis to evaluate some bread wheat genotypes under water deficit conditions. Alexandria Journal of Agricultural Sciences 64:245–256.

https://doi.org/10.21608/alexja.2019.70638

54. Ulukan, H. 2024. Wheat production trends and research priorities: a global perspective. In: Advances in Wheat Breeding: Towards Climate Resilience and Nutrient Security. Springer, Singapore:1–22.

https://doi.org/10.1007/978-981-99-9478-6_1

55. Wang, J.Y., Y.C. Xiong, F.M. Li, K.H. Siddique and N.C. Turner. 2017. Effects of drought stress on morphophysiological traits, biochemical characteristics, yield, and yield components in different ploidy wheat: a meta-analysis. Advances in Agronomy 143:139–173.

https://doi.org/10.1016/bs.agron.2017.01.002

56. Wen, P., Y. Meng, C. Gao, X. Guan, T. Wang and W. Feng. 2023. Field identification of drought tolerant wheat genotypes using canopy vegetation indices instead of plant physiological and biochemical traits. Ecological Indicators 154:110781.

https://doi.org/10.1016/j.ecolind.2023.110781

57. Yadav, S., V. Singh, H. Kesh, A. Naruka, M. Kumar, V.S. Mor and S. Yashveer. 2024. Morpho-physiological characterization and selection of heat tolerant wheat lines using selection indices. Electronic Journal of Plant Breeding 15:347–355.

https://doi.org/10.37992/2024.1502.056

Downloads

Published

2026-03-30

How to Cite

Habib Ur Rehman, Khan, N., Khan, J., Reki, A. R., Rasool, G., Ahmed, S. R., Hafeezullah, Amanullah, Akhtar, H., & Muhammad Ilyas. (2026). Identification of Drought Tolerant Wheat Genotypes through Multi-Index Approach under Different Irrigation Regimes. Indus Journal of Bioscience Research, 4(3), 62-71. https://doi.org/10.70749/ijbr.v4i3.2994