Molecular Analysis of BMP4 Gene Polymorphisms in the Kamori Goat Breed of Khairpur, Sindh Pakistan

Abstract

Bone morphogenetic protein-4 (BMP-4) is a gene that plays a crucial role in the development and growth of various tissues in goat such as it is involved in the formation of the embryo particularly in the development of the mesoderm, which gives rise to muscles, bones and other connective tissues. BMP4 has been linked to reproductive traits in goats such as fertility and little size and development of hair, muscles and fat. BMP4 also involved in the development of various organogenesis such as heart, liver, lungs and kidney. It also play regulates the development of the nervous system. The motive of this research was to investigate the novality of SNPs in BMP-4 gene that have major effect on body growth development. The blood samples were taken from the Animal’s Hospital of district Khairpur by using hygienic protocol and techniques. The blood tubes were transported into dry ice containing cooling box to Molecular Genetic laboratory of Department of Zoology for further process. In the first step DNA extraction from whole blood samples had been done by using of DNA extraction commercial kit and visualized on gel electrophoresis. Later on the PCR products were sent for DNA sequencing for findings of SNPs. The analysis shown that about 08 missense mutation were found in the Kamori goat breed. This mutations were found on the different base pairs (bp). While changes into genetic codon lead to changes into non-essential amino acids into essential amino acids which leads to enhancement of milk and meat qualities and quantities that was very good sign. The result suggested that Kamori goat breed can be implied for breed admixture and genetic marker assist for the selection of breeding.